Protective effect of telmisartan on endoplasmic reticulum stress-related kidney cells apoptosis in diabetic rats
10.3760/cma.j.issn.1000-6699.2011.10.017
- VernacularTitle:替米沙坦对糖尿病大鼠肾脏组织中内质网应激介导相关细胞凋亡的保护作用
- Author:
Yufeng CHEN
;
Huijuan ZHANG
;
Yufen SHEN
;
Wei LIU
;
Jie HE
;
Fengli LI
- Publication Type:Journal Article
- Keywords:
Diabetic nephropathy;
Endoplasmic reticulum stress;
Apoptosis;
Telmisartan
- From:
Chinese Journal of Endocrinology and Metabolism
2011;27(10):849-852
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of angiotensin receptor blocker ( ARB ) telmisartan on endoplasmic reticulum stress -related kidney cells apoptosis in diabetic rats.31 male SD rats were divided into 3 groups:normal control group,diabetic group,and telmisartan treated diabetic group.After 12 weeks' experiment,weight,24 h urinary albumin excretion,blood glucose,serum insulin,and serum creatinine were determined.Renal cell apoptosis was examined by using TUNEL; Renal cell endoplasmic reticulum stress signaling pathway molecules glucose regulated protien 78 ( GRP78 ),caspase12,and CHOP were detected with immunohistochemistry and realtime PCR.24 h urine protein and serum creatinine in diabetic group were significantly higher than those in the control group; while body weight and plasma insulin were significantly lower than those in the control group( P＜0.05 ) ;Compared with diabetic group,24 h urinary protein and serum creatinine were decreased significantly in telmisartan treated diabetic group( P＜0.05 ).The apoptosis index of telmisartan treated group was significantly lower than that of diabetic group( P＜0.05 ).Endoplasmic reticulum stress signaling pathway molecules GRP78,caspase12,CHOP protein and mRNA expression in diabetic group were significantly higher than those in control group( P＜0.05 ),as well as in telmisartan treated group( P＜0.05 ).Endoplasmic reticulum stress took part in kidney cell apoptosis of the diabetic rat.Telmisartan possessed a protective effect on endoplasmic reticulum stress-related renal cells apoptosis.
