Effect of naloxone on L-type calcium current in isolated rat ventricular myocytes

VernacularTitle:纳洛酮对大鼠心室肌细胞L型钙电流的影响
Author: Qunchao WANG ; Qingping WEN
Publication Type:Journal Article
Keywords: Naloxone; Calcium channels,L-type; Myocytes,cardiac
From: Chinese Journal of Anesthesiology 2011;31(8):926-928
CountryChina
Language:Chinese
Abstract: ObjectiveTo evaluate the effect of naloxone on L-type calcium current (ICa-L) in isolated rat ventricular myocytes.MethodsAdult SD rats of both sexes aged 8 weeks weighing 200-250 g were used in this study.Single cardiac ventricular myocytes were enzymatically isolated from SD rats.ICa-L was measured in ventricular myocytes and recorded using whole cell patch-clamp technique.Different concentrations (20 and 100 μg/ml) of naloxone were added to the cardiomyocytes.The effect of naloxone on ICa-L was evaluated.ResultsThe peak current of ICa-L Was inhibited by naloxone in a concentration-dependent manner.Naloxone had no significant effect on steady-state activation curve.ConclusionNaloxone inhibits the L-type calcium channel of ventricular myocytes and exerts negative effect on ventricular muscle function.