Effect of cholinesterase inhibitor on endotoxin-induced brain injury in rabbits
10.3760/cma.j.issn.0254-1416.2011.08.027
- VernacularTitle:胆碱酯酶抑制剂他克林对兔内毒素性脑损伤的影响
- Author:
Bojie WANG
;
Yue SU
;
Hui ZHENG
;
Zhengfang HU
;
Jinzhu WANG
- Publication Type:Journal Article
- Keywords:
Cholinesterase inhibitors;
Endotoxemia;
Brain injuries
- From:
Chinese Journal of Anesthesiology
2011;31(8):998-1000
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of cholinesterase inhibitor on endotoxin-induced brain injury in rabbits.Methods Twenty-one healthy male rabbits were randomly assigned into three groups ( n ＝ 7each):group sham operation (group S),lipopolysaccharide (LPS) group and cholinesterase inhibitor (tacrine hydrochloride,THA) group.LPS 200 μg/kg was intracerebroventricularly injected in LPS group,LPS 200μg/kgand tacrine hydrochloride 150 μg/kg were injected in THA group,while same volume of normal saline was injected in S group.Then blood and tissue samples were collected in different groups after 4 hours.Nuclear factor-kappa B (NF-κB) p65 activity of brain tissues was determined by using Western blot analysis.Tumor necrosis factor-alpha (TNF-α) levels in plasma,cerebrospinal fluid and brain tissues were measured using enzyme linked immunosorbent assay.The brain tissue's myeloperoxidase (MPO) activity and the ratio of wet to dry weight (W/D) were also analyzed.ResultsAs compared with S group,TNF-α level in plasma,cerebrospinal fluid and brain tissues,NF-κB p65 level,MPO activity and W/D ratio increased in LPS and THA groups (P < 0.05).When compared with LPS group,TNF-α level in plasma,cerebrospinal fluid and brain tissues,NF-κB p65 level,MPO activity and W/D ratio decreased in THA group ( P < 0.05 ).ConclusionCholinesterase inhibitor can attenuate the endotoxin-induced brain injury through inhibiting local inflammatory responses.
