Effect of sevoflurane anesthesia in the neonatal period on cognitive function and acetylcholinesterase activity in hippocampus in developmental stages in rats
10.3760/cma.j.issn.0254-1416.2011.08.016
- VernacularTitle:新生大鼠七氟醚麻醉对发育期认知功能和海马乙酰胆碱酯酶活性的影响
- Author:
Guiping XU
;
Dongmei TANG
;
Yongjie ZHANG
- Publication Type:Journal Article
- Keywords:
Anesthetics,inhalation;
Anesthesia;
Infant,newborn;
Child development;
Cognitiondisorders;
AcetylchoLinesterase;
Hippocampus
- From:
Chinese Journal of Anesthesiology
2011;31(8):961-963
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of sevoflurane anesthesia in the neonatal period on cognitive function and acetylcholinesterase(AChE) activity in hippocampus in developmental stages in rats.Methods Eighty 7 day-old SD rats weighing 12-16 g were randomly divided into 5 groups( n ＝ 16 each):groups A and B inhaled 3 ％ sevoflurane in oxygen for 6 and 2 h respectively; groups C and D inhaled 1.5 ％ sevoflurane in oxygen for 6 and 2 h respectively; group E inhaled oxygen only.The cognitive function was assessed using Morris water maze test in weaning period(16-21 day-old) and sexual maturity period (55-60 day-old) respectively.The rats were sacrificed at 30 min after Morris water maze test was finished at 21 and 60 d after birth,and the hippecampus were removed for determination of AChE activity using colorimetry method.ResultsCompared with group E,escape latency was prolonged during 17-20 d after birth in group A,and at 18,19 d after birth in groups B and C,and at 18 d after birth in group C,and AChE activity increased at 21 d after birth in group A( P < 0.05 or 0.01 ).Compared with group A,escape latency was shortened at 19,20 d after birth,and AChE activity decreased at 21 d after birth in groups B and C( P < 0.05 or 0.01).Compared with groups B and C,escape latency was shortened at 19,20 d after birth in group D( P < 0.05).There were no significantly differences in probe time in original platform quadrant,frequency of crossing the original platform and AChE activity at 60 d after birth and escape latency during sexual maturity period among the 5 groups( P ＞ 0.05).ConclusionSevoflurane anesthesia in the neonatal period can reversible decrease the congnitive function in developmental stages in rats in concentration and time dependent manners,and the mechanism may be related to increasing the AChE activity in hippocampus.
