Effects of intrathecal Ro25-6981 on focal cerebral ischemia-reperfusion injury in rats
10.3760/cma.j.issn.0254-1416.2011.08.021
- VernacularTitle:蛛网膜下腔注射R025-6981对大鼠局灶性脑缺血再灌注损伤的影响
- Author:
Fang YAN
;
Yanbing ZHANG
;
Lina WANG
;
Jianping YANG
;
Ying ZHAN
- Publication Type:Journal Article
- Keywords:
Receptors,N-methyl-D-aspartate;
Injections,spinal;
Reperfusion injury;
Brain
- From:
Chinese Journal of Anesthesiology
2011;31(8):977-979
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of intrathecal (IT) Ro25-6981 (a selective NR2B receptor antagonist) on focal cerebral ischemia-reperfusion (I/R) injury in rats.MethodsSixty healthy male SD rats weighing 280-320 g in which IT catheter was successfully implanted without complication were randomly divided into 4 groups ( n ＝ 15 each):group sham operation (group S) ; group focal cerebral I/R(group I/R) ; group I/R + Ro25-6981 (group Ro) and group I/R+ normal saline (group NS).The right middle cerebral artery was occluded for 2 h with a nylon thread with rounded tip which was inserted into right internal carotid artery and advanced cranially until resistance was met in groups I/R,Ro and NS.In group Ro Ro25-6981 100 μg/10 μl was injected IT at 0,2,23 h of reperfusion,while in group NS NS was injected IT instead of Ro25-6981.Neurological dificit was assessed and scored (0 ＝ no deficit,3 ＝ severe deficit) at 24 h of reperfusion.The infarct size was determined by 2,3,5-triphenyl tetrazolium chloride staining.ResultsIT administration of Ro25-6981 significantly reduced cerebral I/R-induced infarct sized and neurological deficit score in group Ro as compared with groups I/R and NS.ConclusionRo25-6981 injected IT can protect the brain against local cerebral I/R injury.
