Effects of leukocyte-depleted allogeneic blood transfusion on perioperative cellular immunity in children
10.3760/cma.j.issn.0254-1416.2011.07.011
- VernacularTitle:滤除白细胞异体血对围术期患儿细胞免疫功能的影响
- Author:
Zhun XING
;
Qiushi WANG
;
Yanni DIAO
;
Qiaoni YANG
;
Xiaojing DUAN
;
Simeng WU
;
Lingxin MENG
- Publication Type:Journal Article
- Keywords:
Leukspheresis;
Blood transfusion;
Immunity,cellular;
Child
- From:
Chinese Journal of Anesthesiology
2011;31(7):816-818
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of leukocyte-depleted allogeneic blood transfusion on perioperafive cellular immunity in children.MethodsThree hundred and fifty-nine ASA Ⅰ or Ⅱ children aged 3 month-14 yr,weighing 5-74 kg requiring allogeneic blood transfusion during operation were randomly divided into 2 groups:control group (group C,n =163) and leukocyte depletion group (group D,n =196).In group D allogeneic blood was filtered with a leukocyte filter before being transfused during operation.Blood samples were collected from peripheral vein before blood transfusion,and at 2 and 6 d after blood transfusion for determination of levels of CD3+,CD4+,CD8+,and CD56+ by flow cytometry.CD4+/CD8+ ratio was calculated.The volume of allogeneic blood transfused during operation,the duration of operation,postoperative drainage,antibiotics administration and hospital stay and incidence of postoperative infection were recorded.ResultsThe levels of CD3+,CD4+,CD56+ and CD4+/CD8+ ratio were significantly increased at 6 d after blood transfusion while the duration of postoperative drainage,postoperative antibiotics administration and hospital stay and incidence of postoperative infection were significantly decreased in group D compared with group C.ConclusionLeukocyte-depleted allogeneic blood transfusion is helpful in improving the postoperative cellular immunity in children.
