Characterization of resistance to β-lactams in clinical isolates of Klebsiella pneumoniae
10.3760/cma.j.issn.1674-2397.2011.05.008
- VernacularTitle:肺炎克雷伯菌临床分离株β-内酰胺类耐药特征分析
- Author:
Jianming ZHU
;
Rujin JIANG
;
Kangle WU
;
Zhaolong MA
;
Haishen KONG
;
Rong ZHANG
;
Huoxiang Lü
;
Zhimi HUANG
;
Changgui SUN
- Publication Type:Journal Article
- Keywords:
Klebsiella pneumoniae;
β-lactamase genes;
Mobile genetic dements;
Index cluster analysis
- From:
Chinese Journal of Clinical Infectious Diseases
2011;04(5):278-283
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate correlation between drug-resistance related genes and mobile genetic elements of Klebsiella pneumoniae resistant to β-lactams. Methods Forty-seven strains of multidrug-resistant Klebsiella pneumoniae were collected from 6 hospitals in Hangzhou and Huzhou of Zhejiang province from August 2008 to May 2010.Modified Hodge test was performed to detect phenotypes of carbapenemases.Forty kinds of β-lactamases (class A-D),ompK35,ompK36,and 12 kinds of mobile genetic elements were detected by PCR,and the results were analyzed by index cluster.ResultsThirty-five strains were positive in modified Hodge test,and 5 kinds of β-lactamases gene ( including KPC-2-like,GenBank:HQ258934) and 9 kinds of mobile genetic elements were detected.Mutations were observed in ompK35 and ompK36 when compared with sensitive strains.Index cluster analysis showed that correlation existed between KPC-2,KPC-2-like and ISKpn6,between TEM-1 and ISEcpl,IS26,int Ⅰ 1,trbC,IS903,and between CMY-2,OXA-30,DHA-1 and tnpU,tnp513,trbC.ConclusionsFive kinds of β-1actamases genes,and mutations in ompK35 and ompK36 may be associated with the resistance to β-1actams in multidrug-resistant Klebsiella pneumoniae.
