Effects of diazoxide pretreatment on expression of phosphatidylinositol 3-kinase mRNA and protein serine/ threonine kinase mRNA in rat myocardial microvascular endothelial cells exposed to hypoxia-reoxygenation
10.3760/cma.j.issn.0254-1416.2011.07.028
- VernacularTitle:二氮嗪预先给药对大鼠心肌微血管内皮细胞缺氧复氧时PI3K mRNA和Akt mRNA表达的影响
- Author:
Su CAO
;
Qiuping CHEN
;
Huanju KANG
;
Shiren SHEN
- Publication Type:Journal Article
- Keywords:
Diazoxide;
Heart;
Endothelium,vascular;
Oxygen;
Cell hypoxia;
1-Phosphatidylinositol 3-kinase;
Protein-serine-threonine kinases;
RNA,messenger
- From:
Chinese Journal of Anesthesiology
2011;31(7):871-873
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of diazoxide pretreatment on expression of phosphatidylinositol 3-kinase(PI3K) mRNA and protein serine/threonine kinase(Akt) mRNA in rat myocardial microvascular endothelial cells exposed to hypoxia-reoxygenation (H/R).MethodsThe SD rat myocardial microvascular endothelial cells were cultured.The cells were seeded in 96-well plates ( 100μd/hole) or in 6 cm diameter dishes (2 ml/dish) with the density of 1 × 106/ml and randomly divided into 4 groups ( n =25 each):normal control group (group C),H/R group,diazoxide pretreatment group (group DZ) and diazoxide pretreatment + 5-hydroxydecanoate (5-HD,a mitochondrial ATP-sensitive potassium channel blocker) group (group DZ + 5-HD).The cells were exposed to 2 h hypoxia followed by 2 h reoxygenation.Diazoxide 100 μmol/L and diazoxide 100 μmol/L + 5-HD 100 μmol/L were added to the culture medium 2 h before hypoxia in groups DZ and DZ + 5-HD respectively.The cell viability,apoptotic rate and expression of PI3K mRNA and Akt mRNA were detected at the end of reoxygenation.ResultsCompared with group C,the cell viability was significantly decreased,while the apoptotic rate increased and expression of PI3K mRNA and Akt mRNA up-regulated in group H/R (P ＜0.05 or 0.01).Compared with group H/R,the cell viability was significantly increased,while the apoptotic rate decreased and expression of PI3K mRNA and Akt mRNA up-regulated in group DZ (P ＜ 0.05 or 0.01).5-HD could inhibit diazoxide pretreatment-induced changes mentioned above (P ＜ 0.05 or 0.01 ).ConclusionDiazoxide pretreatment can reduce H/R injury by promoting PI3K gene and Akt gene transcription through activation of mitochondrial ATP-sensitive potassium channels in rat myocardial microvascular endothelial cells.
