Radiosensitivity enhancement of recombimant adenoviral-mediated retinoblastoma 94 gene in human esophageal carcinoma cells
10.3760/cma.j.issn.0254-5098.2011.05.00
- VernacularTitle:腺病毒介导的人视网膜母细胞瘤94基因对人食管癌细胞辐射增敏作用的研究
- Author:
Fenghua CHEN
;
Jin LI
;
Heng ZHANG
;
Xinru WANG
;
Ying SHAO
;
Lifen WU
;
Enhai JIANG
;
Qin WANG
- Publication Type:Journal Article
- Keywords:
Retinoblastoma 94 gene;
Gene therapy;
Radiation therapy;
Esophageal carcinoma
- From:
Chinese Journal of Radiological Medicine and Protection
2011;31(5):515-518
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the radiosensitivity enhancement effects of recombinant adenovirus mediated retinoblastoma 94 gene on the growth of esophageal carcinoma cells EC109.Methods EC109cells was transfected with recombinant Rb94 gene adenovirus and irradiated by 137Cs γ-rays.The cohorts were divided into groups as blank control,Ad-LacZ,Ad-Rb94,radiation and Ad-Rb94 combined with radiation.Cell inhibition ratio,cell cycle and expression of retinoblastoma protein of EC109 cells were analyzed.Results The growth of EC109 cells transfected with Ad-Rb94,radiation and Ad-Rb94 combined with radiation group was all inhibited.The group of Ad-Rb94 combined with radiation resulted in greater inhibition of cells growth compared with Ad-Rb94 group and radiation group ( F =23.31,P ＜0.05 ).Cells of G2 phases of EC109 cancinoma cells for Ad-Rb94 combined with radiation group were the highest,which was 50％.The combination of Ad-Rb94 and radiation group resulted in the greatest expression of retinoblastoma protein,which reached 71％,significantly higher than Ad-Rb94 infection and radiation groups ( x2 =8.31,6.73,P ＜ 0.05 ).Conclusions Retinoblastoma 94 gene combined with ionizing radiation can enhance the radiation sensitivity of EC109 cells.
