Expression of bepatocyte growth factor activator inhibitor-1 gene in the tissues of renal cell carcinoma and the correlation with tumor angiogenesis
10.3760/cma.j.issn.1673-4904.2011.29.001
- VernacularTitle:肝细胞生长因子激活物抑制因子-1基因在肾癌组织中的表达及其与肿瘤血管生成的关系
- Author:
Chuanyi HU
;
Xiuheng LIU
;
Minghuan GE
;
Chao SONG
- Publication Type:Journal Article
- Keywords:
Kidney neoplasms;
Neovascularization,pathologic;
Hepatocyte growth factor activator inhibitor-1
- From:
Chinese Journal of Postgraduates of Medicine
2011;34(29):1-4
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of hepatocyte growth factor activator inhibitor- 1 (HAl-1) gene in the tissues of renal cell carcinoma (RCC) and the correlation with tumor angiogenesis.Methods The expression of HAI-1 gene was detected by semi-quantitative RT-PCR in the tissues of 56 cases of RCC,corresponding adjacent non-cancer tissues and 12 nomad renal tissues,the expression of CD34was detected by immunohistochemistry SP method in the above-mentioned organizations,and counted the microvessel density (MVD),analyzed the relationship between the expression of HAI-1 gene in RCC and clinicopathological parameters,MVD.Results The positive expression rate of HAI-1 gene in RCC tissues,the adjacent non-cancer tissues and normal renal tissues were 58.93％(33/56),76.79％(43/56) and 91.67％(11/12),respectively.The expression of HAI-1 gene was a negative correlation to the Fuhrman histological grade,Robson clinical stage (r ＝ -0.617,-0.432,P ＜ 0.01 or ＜ 0.05 ).The positive expression rate in lymph node metastasis was significantly lower than that in without lymph node metastasis [ 35.29％(6/17) vs.69.23％(27/39),P＜ 0.05 ].The MVD in RCC tissues was significantly higher than that in the adjacent noncancer tissues and normal renal tissues(P＜ 0.05).The expression of HAI-I gene in RCC tissues was negative correlated to the MVD (r ＝ -0.516,P ＜ 0.01 ).Conclusions There is a certain relationship between the missing expression of HAI-1 gene in RCC and the development,invasion and metastasis of RCC.The HAI-1 gene deletion can be used as an important molecular marker in the invasion and metastasis of RCC.
