Effect of elemene on radiosensitivity of A549 cells and its possible molecular mechanism
10.3760/cma.j.issn.0254-5098.2011.05.003
- VernacularTitle:榄香烯乳对人肺腺癌A549细胞放射敏感性影响及其机制的研究
- Author:
Zhuo ZHANG
;
Lijuan ZOU
;
Yingying TIAN
- Publication Type:Journal Article
- Keywords:
Elemene;
Radiosensitivity;
A549 cells;
DNA-PKcs;
Bcl-2;
P53 protein
- From:
Chinese Journal of Radiological Medicine and Protection
2011;31(5):519-522
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of elemene on the radiosensitivity of A549 cells and its possible molecular mechanism.Methods The effect of radiosensitivity was detected by colony forming assay.The protein expressions of DNA-PKcs,Bcl-2 and P53 were detected with Western blot.The correlation between the protein expression of DNA-PKcs and Bcl-2,DNA-PKcs and P53 was analyzed.Results Elemene had radiosensitizing effect on A549 cells,with the SERDo and SERDq 1.54 ± 0.20 and 1.43±0.15,respectively for 10 μg/ml elemene,and 1.63 ±0.32 and 1.75 ±0.19,respectively for 20 μg/ml elemene.Compared with irradiation group,the expression of DNA-PKcs was reduced significantly in 10,20 μg/ml elemene combined with radiation group ( t =7.52,8.33,P ＜ 0.05 ),so was for Bcl-2(t =10.74,11.33,P ＜0.05).The expression of P53 protein increased significantly (t =-9.25,7.66,P ＜0.05).There was a remarkable negative correlation between the expression of DNA-PKcs and P53(r =-0.569,P ＜0.05),and a remarkable positive correlation between DNA-PKcs and Bcl-2 (r =0.755,P ＜0.05 ).Conclusions Elemene has radiosensitizing effect on A549 cells,which might be related to down-regulation of DNA-PKcs gene expression,up-regulation of P53 and down-regulation of Bcl-2.
