Effects of tetramethylpyrazine on neuropafhdc pain in rats
10.3760/cma.j.issn.0254-1416.2011.07.019
- VernacularTitle:川芎嗪对大鼠神经病理性痛的影响
- Author:
Xiangmei GAO
- Publication Type:Journal Article
- Keywords:
TETRAMETHYLPYRAZINE;
Neuralgia
- From:
Chinese Journal of Anesthesiology
2011;31(7):840-843
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo evaluate the effects of tetramethylpyrazine on neuropathic pain (NP) in rats.MethodsFifty-four male SD rats weighing 180-220 g were randomly divided into 3 groups ( n =18 each):sham operation group (group S); group NP and tetramethylpyrazine group (group T).NP was induced by chronic constrictive injury (CCI) in groups NP and T.Four ligature were placed on right sciatic nerve at 1 mm intervals with 4-0 chromic catgut.In group T tetramethylpyrazine 100 mg/kg was administered intraperitoneally once a day for 14 consecutive days after operation.Thermal and mechanical pain threshold was measured at 1 day before and 3,7,and 14 d after operation.Six animals in each group were sacrificed after pain threshold measurement on the 3rd,7th and 14th days after operation and the lumbar segment ( L(4).5 ) of the spinal cord was removed for determination of apoptosis (by TUNEL) and Bcl-2 and caspase-3 expression in spinal dorsal horn (by immuno-histochemistry).Apoptosis rate (AR =the number of apoptotic cells/the total number of cells examined) was calculated.Results CCI significantly decreased the thermal and mechanical pain threshold,up-regulated Bcl-2 and caspaas-3 expression in spinal dorsal horn and increased AR in groups NP and T.Tetramethylpyrazine significantly increased the thermal and mechanical pain threshold,up-regulated Bcl-2 expression and down-regulated caspase-3 expression and decreased AR in group T as compared with group NP.ConclusionTetramethylpyrazine can attenuate NP through inhibition of apoptosis in the spinal cord.
