Experimental study of dexamethasone in the prevention of delayed encephalopathy after carbon monoxide poisoning
10.3760/cma.j.issn.1671-0282.2011.11.003
- VernacularTitle:地塞米松预防大鼠一氧化碳中毒迟发性脑病的疗效
- Author:
Qin LI
;
Ying DENG
;
Kuang FU
;
Haibo LAN
;
Xinchun WANG
;
Fengping WANG
- Publication Type:Journal Article
- Keywords:
Dexamethasone;
Rat;
Carbon monoxide poisoning;
Delayed encephalopathy;
Demyelination;
Myelin basic protein;
Autoimmune response;
Magnetic resonance imaging
- From:
Chinese Journal of Emergency Medicine
2011;20(11):1131-1134
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the curative effects of dexamethasone in the prevention of delayed encephalopathy after acute carbon monoxide poisoning in the rats.Methods Eighty healthy male Wistar rats were randomly(random number)divided into hyperbaric group(H),dexamethasone group (D),combined treatment group(C)and model control group(M)after carbon monoxide poisoning,twenty rats in each group.Twelve air-modeling rats were selected as normal control group(N).Using eight-arm maze training and testing,the rat's function of cognitive and memory was detected.The serum MBP levels were detected by enzyme-linked immunosorbent assays(ELISA).Magnetic resonance imaging was used for observing the demyelination of the head and the morbidity of delayed encephalopathy.Measured data was analyzed with single factor analysis of variance(one-way ANOVA).Results The result of eight-arm maze showed that there were 6,7,1,1 rats with delayed encephalopathy in groups M,H,C,D,respectively.At 3 days after poisoning,except N group,the serum MBP levels of every groups increased significantly.At 10 days after poisoning,serum MBP levels in groups C and D were significantly decreased,then returned to normal levels at day 18.The serum MBP in groups M and H was higher than normal levels at all the time.Head MRI showed except D group,the rest groups were abnormal signals,which appeared 7 in M group,6in H group and 1 in C group.Conclusions Dexamethasone administrated as soon as possibly after acute carbon monoxide poisoning may reduce the serum MBP levels,prevent demyelination occurs,decrease the pathological damage,eventually play a preventive role in DE.
