Antimicrobial Resistance and Molecular Epidemiologic Characteristics of Neisseria gonorrhoeae Isolated from Korea in 2013.
10.5145/ACM.2013.16.4.182
- Author:
Hyo Jin KIM
1
;
Younghee SEO
;
Wan Hee KIM
;
Yangsoon LEE
;
Hyukmin LEE
;
Kyungwon LEE
;
Yunsop CHONG
Author Information
1. Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Microbial sensitivity tests;
Neisseria gonorrhoeae;
Neisseria gonorrhoeae multiantigen sequence typing
- From:Annals of Clinical Microbiology
2013;16(4):182-187
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Antimicrobial resistance of Neisseria gonorrhoeae has become a serious problem worldwide, and ceftriaxone non-susceptible isolates have been recently reported from Japan and Europe. In this study, we evaluated the antimicrobial susceptibilities and molecular epidemiological characteristics of isolates from Korea in 2013. METHODS: Sixty strains of N. gonorrhoeae were collected from Korean patients and prostitutes. Antimicrobial susceptibility was tested by the agar dilution and disk diffusion methods. N. gonorrhoeae multi-antigen sequence typing (NG-MAST) was performed in order to determine the molecular epidemiologic relatedness. RESULTS: All of isolates were non-susceptible to penicillin G and tetracycline, and the rate of ciprofloxacin-resistant isolates was 95% in 2013. The MICs of ceftriaxone were within the susceptible range for all isolates, but one isolate non-susceptible to cefixime (MIC=0.5 microg/mL) was encountered. The isolates with decreased susceptibility (MIC< or =0.12 microg/mL) to cefixime or ceftriaxone accounted for 10% and 14% of the isolates tested, respectively. In NG-MAST analysis, 40 different STs were encountered among the 59 isolates. Isolates that belonged to tbpB110 showed higher cefixime and ceftriaxone MICs (0.12-0.5 microg/mL) as well as cefixime resistance. CONCLUSION: Most of the N. gonorrhoeae isolates showed susceptibility to spectinomycin and cephalosporins. Due to the emergence of isolates that are non-susceptible to cefixime and the prevalence of isolates with the tbpB110 allele belonging to ST1407, which cause cefixime and ceftriaxone treatment failure in successful global clones of N. gonorrhoeae, a continuous nationwide antimicrobial surveillance program is required to monitor the emergence of cephalosporin resistance in N. gonorrhoeae.
