Effect of precondition with Toll-like receptor 4 monoclonal antibody on LPS-induced acute lung injury in mice
10.3760/cma.j.issn.1671-0282.2011.10.012
- VernacularTitle:Toll样受体4单克隆抗体预处理对脂多糖诱发小鼠急性肺损伤的影响
- Author:
Xiaotong CHEN
;
Shouping WANG
;
Zijun ZOU
;
Zhijie HE
- Publication Type:Journal Article
- Keywords:
Acute lung injury;
Sepsis;
Toll-like receptor;
Lipopolysaccharide;
Precondition;
Acute respiratory distress syndrome;
Inflammatory reaction;
Inflammatory factor
- From:
Chinese Journal of Emergency Medicine
2011;20(10):1052-1055
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of precondition with Toll-like receptor 4 monoclonal antibody (TLR4mAb) on lipopolysaccharide (LPS) -induced acute lung injury in mice.Methods A total of 45 male BALB/c mice were randomly divided into 3 groups:the control group ( group C),the sepsis group (group S) and the pretreatment group (group P).Mice in the group P and group S were injected intraperitoneally with LPS ( 10 mg/kg) to produce acute lung injury models.Mice in the group P was injected intraperitoneally with TLR4mAb (5 μg/g) 1 h before the injection of LPS.Expression of TLR4mRNA in lung tissue,expression of TNF-α and IL-6 in serum,water content of lung,and the pathomorphologic changes of lung were detected after 6 h,12 h and 24 h.One-way ANOVA was used for inter-group comparison and two-way ANOVA was used for intra-group comparison.Results Compared to group C,water content significantly increased at 12 h and 24 h in group S and group P; compared to group S,water content significantly decreased in group P at 12 h and 24 h.Compared to group C,the expression of IL-6 and TNF-α significantly increased in group S and group P at 6 h,12 h and 24 h; compared to group S,the expression of IL-6 and TNF-α significantly decreased at 6 h,12 h and 24 h in group P.Compared to group C,the expression of TLR4 mRNA increased significantly in group S and group P at 6 h,12 h and 24 h; compared to group S,the expression of TLR4 mRNA decreased significantly in group P at6 h,12 h and 24 h.Compared to group S,pathological damage of the lung was improved significantly in group P.Conclusions Precondition with TLR4mAb can attenuate LPS-induced acute lung injury,suppress the expression of inflammatory factors.Regulation of TLR4 pathway may be a promising therapeutic strategy for ALI.
