Determination of baicalin in the children's Yffei-Pingchuan mixture by RP-HPLC
10.3760/cma.j.issn.1673-4246.2011.10.012
- VernacularTitle:RP-HPLC法测定小儿益肺平喘合剂中黄芩苷的含量
- Author:
Zhaojun CHEN
;
Zhong LI
;
Hui JIN
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
the children's Yifei-Pingchuan mixture;
Baicalin;
Content determination
- From:
International Journal of Traditional Chinese Medicine
2011;33(10):900-902
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the determination method of baicalin in the children's YifeiPingchuan mixture by RP-HPLC.Methods A Kromasil C18 column was used with methanol-(0.4％)phosphoric acid-triethylamine (45 ∶ 55 ∶ 2 drops) as mobile phase with a column temperature of 28 ℃.The flow rate was 1.0 ml/min.The detection wavelength was set at 277 nm.Results The linear range of baicalin was 0.040～1.000 μg (r=0.9999) with a average recovery of 99.82 and RSD of 1.01％.Conclusion The method for quantification of baicalin was sensitive,accurate,and convenient,which can also be used as one of basis of quality standard for Yifei-Pingchuan mixture.
