Experimental analysis the first cases of cholera epidemic detection of 0139 of Huainan
10.3760/cma.j.issn.1008-6706.2011.16.003
- VernacularTitle:安徽省淮南市首起0139霍乱弧菌疫情检测分析
- Author:
Shoujie YU
;
Xiaomei LIU
;
Min CAO
;
Shoukui HU
;
Wanfu HU
- Publication Type:Journal Article
- Keywords:
Cholera;
Cholera toxin;
Toxin coregulated pilus;
Turtle
- From:
Chinese Journal of Primary Medicine and Pharmacy
2011;18(16):2166-2167
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo identify the first cases of cholera in Huainan,0139 epidemic causes and biological properties. MethodsConventional methods of serological and bacteriological methods of the strains were identified by polymerase chain reaction (PCR) to detect cholera virulence genes, susceptibility testing modified KB.Results1 ,was isolated and serum agglutination results,2 patients were all positive stool samples,6 were in close contact with all the negative rectal swab;turtle pond-like 18,5 were positive;turtle egg in battle were like 9,2 were positive ; turtle eggs 7, the positive three copies; turtle waste 11, were negative. 2, or more positive culture drug sensitivity test of the pioneer B, doxycycline resistant; to ampicillin, tetracycline in sensitive;on norfloxacin, gentamicin, ciprofloxacin sensitive. 3,or more positive cultures gene DNA PCR test results,cholera toxin(CT) ,toxin coregulated pilus (TCP) all were positive. ConclusionFrom the outbreak of cholera 0139 is water pollution caused by turtle ponds,The trip types of 0139 Vibrio cholera were highly homologous with that isolated from green turtle;the bacteria of the pioneer B, doxycycline resistance and carrying cta, tcpa virulence genes.
