MicroRNA-27 Promotes Odontoblast Differentiation via Wnt1 Signaling.
10.11620/IJOB.2015.40.4.197
- Author:
Ji Ho CHO
1
;
Su Gwan KIM
;
Byung Sun PARK
;
Dae San GO
;
Joo Cheol PARK
;
Do Kyung KIM
Author Information
1. Oral Biology Research Institute, Chosun University School of Dentistry, Gwangju 501-759, Republic of Korea. kdk@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
miR-27;
odontoblasts;
differentiation;
Wnt1
- MeSH:
3' Untranslated Regions;
Animals;
Binding Sites;
Cell Differentiation;
Cell Proliferation;
Dental Papilla;
Mice;
MicroRNAs;
Odontoblasts*;
Protein Biosynthesis;
RNA, Messenger;
Up-Regulation
- From:International Journal of Oral Biology
2015;40(4):197-204
- CountryRepublic of Korea
- Language:English
-
Abstract:
MicroRNA (miRNA, miR) is essential in regulating cell differentiation either by inhibiting mRNA translation or by inducing its degradation. However, the role of miRNA in odontoblastic cell differentiation is still unclear. In this study, we examined the molecular mechanism of miR-27-mediated regulation of odontoblast differentiation in MDPC-23 mouse odontoblastic cells derived from mouse dental papilla cells. The results of the present study demonstrated that the miR-27 expression increases significantly during MDPC-23 odontoblastic cell differentiation. Furthermore, miR-27 up-regulation promotes the differentiation of MDPC-23 cells and accelerates mineralization without cell proliferation. The over-expression of miR-27 significantly increased the expression levels of Wnt1 mRNA and protein. In addition, the results of target gene prediction revealed that Wnt1 mRNA has an miR-27 binding site in its 3'UTR, and is increased by miR-27. These results suggested that miR-27 promotes MDPC-23 odontoblastic cell differentiation by targeting Wnt1 signaling. Therefore, miR-27 is a critical odontoblastic differentiation molecular target for the development of miRNA based therapeutic agents in dental medicine.
