HIF-1α expression in lung tissue of rats with paraquat poisoning
10.3760/cma.j.issn.1671-0282.2011.09.013
- VernacularTitle:百草枯中毒大鼠肺组织缺氧诱导因子-1α的表达变化
- Author:
Rong XU
;
Ruilan WANG
;
Xin WU
;
Xue TANG
- Publication Type:Journal Article
- Keywords:
Paraquat;
Poison;
HIF-1α;
Hypoxemia;
Acute lung injury;
Pulmonary interstitial fibrosis;
Alveolar epithelial cell
- From:
Chinese Journal of Emergency Medicine
2011;20(9):945-950
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the HIF-1α expression in rats with paraquat poisoning (PQP).MethodsNinety-six healthy SD rats were randomly (random number) divided into 2 groups ( n =48 in each group) : PQP group and control group. The PQP group was further divided into six subgroups as per thedifferent intervals after Paraquat intoxication. Samples from 6 PQP subgroups and control group were taken at 0 h, and 2 h, 6 h, 12 h, 24 h, 48 h, 72 h and 120 h after paraquat administration. Aarterial blood wascollected for blood gas analysis, and the sections of lung tissue were stained with hematoxyhn-eosin and masson's trichrome to detect the trichrome positive area, and HIF-1α was detected by immunohistochemistryAll data were processed with one-factor analysis of variance and Pearson correlation analysis. ResultsTwo hours after paraquat poisoning, the levels of HIF-1α protein and the collagen were both significantly upregulated in the lung tissue in rats with paraquat poisoning. However, the hypoxemia existed until 72h.There was correlation between levels of Masson's trichrome positive area and HIF-1α positive area (r =0. 819, P ＜0. 05 ). There was correlation between the levels of lactic acid in blood gas analysis and HIF-1α positive area (r=0. 761, P＜0. 05. But there was no correlation between levels ofPO2 and HIF-1α positive area (r=-5.24, P＞0. 05). ConclusionsThe level of HIF-1α was up-regulated in rats with paraquat poisoning and it was not associated with hypoxemla.
