Role of iatrogenic transforming growth factor-β1 antibody in peripheral neural fibrosis after chronic entrapment
10.3760/cma.j.issn.1001-8050.2011.09.015
- VernacularTitle:外源性转化生长因子-β1抗体对周围神经慢性卡压后神经纤维化的缓解作用
- Author:
Rui HU
;
Jie LAO
;
Zhongwei JIA
;
Fanbin MENG
;
Zhenbing CHEN
- Publication Type:Journal Article
- Keywords:
Transforming growth factor-β;
Antibodies;
Nerve compression syndromes;
Fibrosis
- From:
Chinese Journal of Trauma
2011;27(9):816-821
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of transforming growth factor-β1 (TGF-β1) antibody on peripheral neural fibrosis after chronic entrapment.MethodsA total of 75 rats were randomly divided into three groups, ie, Group A (sham operation, only the sciatic nerve exposed), Group B (compression only, treated with sciatic nerve entrapment) and Group C (compression plus antibody injection).Electron microscopy, immunohistoehemistry, RT-PCR and Western blot were performed to observe the morphological changes of the compressed nerve tissue and to determine the level of TGF-β1 , collagen Ⅰ and collagen Ⅲ at 2, 4, 6, 8, 10 weeks after sciatic nerve compression, respectively.Results The levels of TGF-β1 , types Ⅰ and Ⅲ collagen protein were increased significantly in the Group B compared with that in the Group A, when the expression of TGF-β1 was increased in the early phase of the compression, reached the peak at the 4th week, and then decreased slowly.The expressions of collagenⅠand collagen Ⅲ were increased after compression, reached a peak at the 6th week and then maintained a relatively high level.The number of the fibrous tissues was decreased significantly and the content of types Ⅰ and Ⅲ collagen protein declined in the Group C, with statistical difference compared with the Group B (P ＜ 0.05).ConclusionsPeripheral nerve fibrosis can be caused by chronic nerve compression.TGF-β1 plays an important role in effectively inhibiting the collagen synthesis and ameliorating the nervous fibrosis of the protein following peripheral nervous entrapment.
