Revascularization and osteogenesis during repair of bone defects with tissue-engineered bone of nano-hydroaoatite crystal/polyamide 66/rabbit osteoplast/vascular endothelial growth factor
10.3760/cma.j.issn.1001-8050.2011.09.022
- VernacularTitle:纳米羟基磷灰石/聚酰胺66/成骨细胞/血管内皮生长因子165组织工程骨修复骨缺损再血管化与成骨活性
- Author:
Junhua ZHAO
;
Chunyang MENG
;
Dianming JIANG
;
Hong AN
;
Guowu CHEN
- Publication Type:Journal Article
- Keywords:
Hydroxyapatites;
Vascular endothelial growth factors;
Bone defect
- From:
Chinese Journal of Trauma
2011;27(9):852-857
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo employ the vascular endothelial growth factor (VEGF165) gene transfected rabbbit osteoplast to combine with the porous nano-hydrxyapatite crystal (n-HA)/polyam ide 66(/PA66) so as to evaluate the osteogenesis and rapid revascularization early after repair of the rabbit radius bone defects with the tissue-engineered bone (n-HA/PA66/osteoplast/VEGF165).MethodsThe animal models of bilateral radius bone defects were created in 56 New Zealand white rabbits that were then randomly divided into Group A and Group B.In Group A, the animals were implanted with n-HA/PA66on the left bone defects (Group Al) and with n-HA/PA66/VEGF165 composite materials on the right bone defects (Group A2). In Group B, the animals were implanted with n-HA/PA66/osteoplast/VEGF165 composite materials on the left side (Group B1) and with n-HA/PA66/osteoplast on the right side (Group B2).Gross, digital radiography, histological sections, vessel count and scanning electron microscopy (SEM) were performed at 2, 4, 8 and 12 weeks after operation.ResultsThe osteogenesis and revascularization in Group B1 was superior to that in the other groups at each time point, with statistical difference (P ＜0.05).The revascularization and osteogenesis in Groups B1 and B2 was far better than that in Groups A1 and A2, with no statistical difference between Group A1 and Group A2.ConclusionsThe new tissue-engineered bone (n-HA/PA66/osteoplast/VEGF165) has a perfect osteogenetic effect and can promote rapid revascularization and the bone healing in the early stage after repair of the bone defects.
