Apoptotic Effects of 6-Gingerol in Human Breast Cancer Cells.
10.11620/IJOB.2015.40.4.223
- Author:
Hyun Woo KIM
1
;
Deuk Hee OH
;
Jeong Tae KOH
;
Young Chai LIM
Author Information
1. Department of Pharmacology, Chonnam National University Medical School, Gwangju, Korea. limyc@jnu.ac.kr
- Publication Type:Original Article
- Keywords:
6-Gingerol;
Apoptosis;
Caspase-3;
PARP;
Human Breast Cancer Cells
- MeSH:
Apoptosis;
Breast Neoplasms*;
Breast*;
Caspase 3;
Cell Line;
Epithelial Cells;
Flow Cytometry;
Humans*;
MCF-7 Cells
- From:International Journal of Oral Biology
2015;40(4):223-228
- CountryRepublic of Korea
- Language:English
-
Abstract:
6-Gingerol exerts anti-tumor effects in various cancer cell models. We evaluated the effect of 6-gingerol on the growth of MCF-7 breast cancer cells and MCF-10A breast epithelial cells to determine whether any growth-inhibitory effects found were attributable to apoptosis, and to elucidate the underlying mechanism of action. 6-Gingerol inhibited the viability of both cell lines in a dose- and time-dependent manner; however, the degree of inhibition was greater in MCF-7 than MCF-10A cells. By flow cytometry, induction of dose- and time-dependent apoptosis was found, and the magnitude of apoptosis was also markedly greater in MCF-7 than MCF-10A cells. Expression of caspase-3 and poly (ADP-ribose) polymerase (PARP) was observed in MCF-7 cells treated with 6-gingerol, and further cleavage of PARP occurred in these cells. We suggest that 6-gingerol induces apoptosis in human breast cancer cells mainly by promoting caspase-3 expression and subsequent degradation of PARP.
