Effect of hypoxia-inducible factor-1α on proliferation and invasion of prostate cancer PC-3 cell in hypoxic situation

VernacularTitle:缺氧诱导因子-1α对低氧状态下前列腺癌PC-3细胞株增殖及侵袭的影响
Author: Rongfu LIU ; Pengcheng ZHAN ; Boan LI
Publication Type:Journal Article
Keywords: Hypoxia-inducible factor-1α; Hypoxic; Prostate cancer PC-3; Proliferation; Invasion
From: Chinese Journal of Urology 2011;32(9):599-602
CountryChina
Language:Chinese
Abstract: ObjectiveWE transfected the recombinant expression plasmid of pcDNA3. 1-HIF-1α into the prostate cancer cells, to research the effect of HIF-1α on proliferation of prostate cancer cell PC-3.MethodsWe selected a stable expression cell line with G418 were selected by transfection of the recombinant expression plasmid of pcDNA3. 1-HIF-1α into the prostate cancer. The protein and mRNA expression of HIF-1α was assayed by western - blot and RT-PCR. The cells growth curves were described by MTT and the ability of invasion was assayed by Transwell.ResultsThe expression of HIF-1α mRNA was not obviously increased compared to the untransfected prostate cancer cell by RT-PCR, but the expression of HIF-1α protein was up-regulated by western-blot after the recombinant expression plasmid transfected into PC-3. The ability of cell proliferation and invasion was significantly enhanced by MTT and Transwell assays.ConclusionThe stable expression cell model of HIF-1α was successfully constructed, which enhanced the proliferation and invasion of prostate cancer cell PC-3.