Mrp14 is a potential biomarker for biliary tract neoplasms
10.3760/cma.j.issn.1007-8118.2011.09.009
- VernacularTitle:胆道肿瘤的潜在标志物Mrp14
- Author:
Zhihui GAO
;
Tao Lü
;
Houbao LIU
;
Liying WANG
;
Xiliang ZHA
;
Zhilong AI
;
Tao SUO
;
Saixiong TONG
;
Bingsheng WANG
- Publication Type:Journal Article
- Keywords:
Bile duct cancer;
Cholangiocarcinoma;
Gallbladder cancer;
Tumor markers;
Proteomics
- From:
Chinese Journal of Hepatobiliary Surgery
2011;17(9):722-726
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo provide clues to find a biomarker for early diagnosis, prognosis and therapy, as well as to understand the molecular mechanisms governing cancer progression. Methods Surgical specimens were obtained from 87 patients with histopathologically proven malignant or benign lesions. The differential protein profiles of these malignant and benign specimens were detected using two-dimensional electrophoresis (2-DE) combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS). Western blotting and immuno-histochemistry were used to validate the results. RT-PCR was used to detect the gene expression in the tissues. ResultsMrp14 was found to be overexpressed in the tumor tissues of gallbladder cancer and extra-hepatic bile duct cancer, and in the bile of patients with malignant biliary tract tumours. The result was further verified using Western blot and immuno-histochemistry. RT-PCR confirmed the overexpression of Mrpl4 at the gene level. Mrp14 is a potential biomarker for biliary tract neoplasms. ConclusionsThis is the first report which described the overexpression of Mrp14 in biliary tract neoplasms and further studies are needed to confirm our findings. Mrp14 may be a potential hiomarker for biliary tract neoplasms. It may provide important clues on the molecular mechanisms governing cancer progression.
