Effects of suppressing the expression of miRNA-21 on the apoptosis and invasion abilities of cholangiocarcinoma cells and its target gene

VernacularTitle:抑制miRNA-21表达对胆管癌细胞凋亡、侵袭的影响及其靶基因探讨
Author: Hao YOU ; Qiang HUANG ; Chenhai LIU ; Fang XIE ; Kai ZHU
Publication Type:Journal Article
Keywords: Cholangiocarcinoma; miRNA-21; Apoptosis; Invasion; Tumor suppressor gene,RECK
From: Chinese Journal of Hepatobiliary Surgery 2011;17(9):760-763
CountryChina
Language:Chinese
Abstract: ObjectiveTo discuss the effects of apoptosis and invasion of RBE cells caused by miRNA 21 suppression and further investigate the potential role of miRNA-21 plays on target mRNA regulation. MethodsThe RNAi technology was employed to suppress the expression of RBE cells.The changes in RECK mRNA and protein expressions were detected by RT-PCR and Western blotting respectively. Changes occurred in apoptosis was closely monitored by flow cytometry (FCM). The invasion of RBE cells was analyzed in vitro by invasion assay (transwell). ResultsThe expression of miRNA-21 was clearly suppressed while the RECK mRNA and protein were over-expressed. The rate of apoptosis was significantly accelerated and there was a dramatic decrease in RBE cells' ability to invade after miRNA-21 knockdown. ConclusionThrough miRNA-21 suppression, the rate of apoptosis of RBE cells was accelerated whereas their invasion ability was greatly reduced. RECK was found to be the target gene of miRNA-21 which participates in the regulation process of regulation.