Protective effect of high expression of glutathione peroxidase on cell model of Alzheimer disease
10.3760/cma.j.issn.0254-9026.2011.09.018
- VernacularTitle:高表达谷胱甘肽过氧化物酶1对阿尔茨海默病细胞模型的作用
- Author:
Weirui ZHANG
;
Lijun LIU
;
Xiaohong LIU
;
Yongjun HUANG
;
Yuying WU
;
Yanli ZHANG
;
Xinxin CHENG
- Publication Type:Journal Article
- Keywords:
Alzheimer disease;
Glutathion peroxidase;
Dxidative stress
- From:
Chinese Journal of Geriatrics
2011;30(9):766-769
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of eliminating free radical and increasing antioxidative capacity of glutathione peroxidase 1(GPX1) on PC12 cells. MethodsGPX1 recombinant plasmid and Plncxplasmid were transfected into PC12 cells and PC12 cells highly-expressing GPX1 stably were sieved by G418 solution. PC12 ceils were treated with different concentrations of amyloid β-protein (Aβ25-35) for 48 h, to decide the optimal concentration of Aβ25-35 and construct ideal cell model. GPX1/pLNCX/PC12 group, pLNCX/ PC12 group and PC12 group were treated with optimal concentration of Aβ25-35 ,respectively for 48 h, and their absorbance (A) value by MTT conversion was compared among three groups.ResultsCell clone highly expressing GPX1 stably were obtained by G418 selection. The increment of cell inhibition ratio was 24.7 ％ after 20 μmol/L Aβ25-35 treatment for 48 h, compared with control group (P＜0.01). Thus the optimization concentration of Aβ25-35 was 20 μmol/L. After treatment with 20 μmol/L for 48 h, the A value was significantly higher in GPX1/pLNCX/PC12 group than in pLNCX/ PC12 group and in PC12 cells group(0.53±0. 02 vs. 0.44±0.02;0.53±0.02 vs. 0.39±0.07, P＜0.01). Conclusions Transfection of GPX1 recombinant plasmid may protect cell against injury from free radical and reverse the decrease of PC12 cell survival rate impaired by Aβ25-35.
