Dynamic changes of renal blood perfusion in Goto-Kakizaki rats using contrast-enhanced ultrasonography
10.3760/cma.j.issn.1004-4477.2011.09.027
- VernacularTitle:超声造影增强显像对自发性糖尿病大鼠肾血流灌注的实验研究
- Author:
Fang MA
;
Baozhen ZHAO
;
Yuanyuan LIU
;
Yuanyuan DANG
;
Weiping LI
;
Lehang GUO
;
Chen Lü
;
Rong WU
;
Lin YANG
;
Wei YAN
;
Xiao HUANG
;
Chaoqing WANG
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Microbubbles;
Diabetes mellitus;
Kidney;
Hemodynamic phenomena
- From:
Chinese Journal of Ultrasonography
2011;20(9):803-807
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore dynamic changes of renal cortex blood perfusion in Goto-Kakizaki rats(GK rats) by contrast-enhanced ultrasonography.The clues for early diagnosis and intervention of diabetic renal impairment may be obtained from the study.Methods Male GK rats were taken as experimental groups,and Wistar male rats were used as controls.Contrast-enhanced agent SonoVue was administered via the femoral vein cannulation in a bolus fashion.Image changes of renal cortex were observed in real time, and dynamic image changes within 0 - 5 minute after injection were observed and stored.The renal cortex blood perfusion changes were analyzed using the time-intensity curve (TIC).Results (1) The kidney was quickly enhanced after the injection of contrast agent.The renal artery, cortex, renal vein were displayed in sequence.(2)The shape of renal perfusion TIC in all the objects was a single peak,steep ascending, flat descending curve.The overall shape of TIC in GK rats was wider than controls and they were wider and wider with age increasing of the GK rats.(3) Compare to controls,the time to peak (TTP)and the half time of peak descending (HDT) in GK rats was longer (P ＜0.01), the slope rates of the ascending curve (S1) and the slope rates of the descending curve (S2) decreased and the area under the curve increased in 12-week-old and 20-week-old GK rats (P ＜ 0.05 or P ＜ 0.01).Conclusions The abnormal blood perfusion of renal cortex microcirculation was present in the early stage of diabetic in GK rats.Real-time contrast-enhanced ultrasonography and TIC could display this change effectively.
