Photodynamic therapy-induced the apoptosis of ovarian and cervical cancer cell lines in vitro studies
10.3760/cma.j.issn.0254-9026.2011.09.021
- VernacularTitle:光动力学疗法诱导卵巢癌细胞系Skov3和宫颈癌细胞系Hela凋亡的体外研究
- Author:
Mao YING
;
Shen KENG
;
Wei WEI
- Publication Type:Journal Article
- Keywords:
Photochemotherapy;
Apoptosis;
Ovarian neoplasms;
Uterine cervical neoplasms
- From:
Chinese Journal of Geriatrics
2011;30(9):779-783
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveThe apoptosis and anti-proliferation effects of photodynamic methods on cervical cancer and ovarian cancer cell lines were studied to explore the advantage of photodynamic therapy in gynecological cancer.MethodsCervical cancer cell line (Hela) and ovarian cancer cell line (SK()V3) were treated with photosensitizer (5-ALA) at different doses and then radiated by laser with energy scopes. The morphological characteristics were observed by light microscopy and electronic microscopy. Inhibition effects and cell apoptosis were studied by MTT assay and flow cytometry (FCM) ResultsThe cytomorphosis and shrinkage, nucleus condensation and anachromasia, dense chromatin lining along cell membrane were observed in both cell line receiving photodynamic therapy.Through transmission electron microscope (TEM), we found nucleus chromatin gathering along membrane of cell nuclear and apoptotic body formed. The cell inhibition rates were 12.9％-90.7％ and 3.9 ％-70.0％, respectively in Skov3 cell and HeLa cell treated with 5-ALA of different concentrations (0.1, 0.5, 1.0, 2.5 mol/L) and receiving laser of different doses (0.1,0.5, 2.5, 12.5 J/cm2). The concentration of the photosensitizer and the dose of laser influenced the optical density(A) of cell lines (P＜0.01). The photo sensitizer was related strongly to laser (P＜0.01). The optical density of the two cell lines decreased with the increases of photo sensitizer concentration and laser dose ( P＜0.01 ),showing concentration- and time-dependent manner.Conclusions Photodynamic therapy inhibits the growth of ovarian cancer and cervical cancer cell line.
