Observation of urokinase targeted microbubbles dissolved the thromb in vivo by electron microscope
10.3760/cma.j.issn.1004-4477.2011.09.025
- VernacularTitle:超声介导下携尿激酶靶向微泡在兔动脉内溶栓后的电镜观察
- Author:
Lina GUAN
;
Chunmei WANG
;
Yuming MU
;
Zhisheng WU
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Mierobubbles;
Urinary plasminogen activator;
Thrombolytic therapy
- From:
Chinese Journal of Ultrasonography
2011;20(9):799-802
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the change of thrombosis in rabbits arteries by electron microscope after thromb was lyses by urokinase targeted microbubbles.Methods A total of 24 rabbits with plateletrich thrombi in the femoral artery were randomized into 4 treatment groups (n = 6) :(1) ultrasound alone (US) ; (2) urokinase alone (UK); (3) ultrasound, non-targeted microbubble and urokinase (US + M + UK) ;(4) ultrasound, platelet-targeted microbubble and urokinase (US + R + UK).Urokinase targeted microbubbles were in conjunction with the surface of commercial microbubbles (SonoVue) by direct conjugation method.US was simultaneously applied transcutaneously over the thrombus up to 30 min.The thrombolytic effect was based on ultrasound, blood flow and histological observations at 120 min post treatment.Results US + R + UK group was completed recanalization (P ＜ 0.001).Scanning electron microscope examination showed thrombosis of the fiber network structure damage.Conclusions The main electron microscope change of RGDS urokinase targeted microbubbles dissolve thromb is thrombus fibrin network structure damage and fibrin dissolution.
