Expression and clinical significance of HA117 in adults with acute leukemia
10.3760/cma.j.issn.1673-422X.2011.08.025
- VernacularTitle:HA117在成人急性白血病中的表达及意义
- Author:
Xia WU
;
Junhe WANG
- Publication Type:Journal Article
- Keywords:
Leukemia;
Drug resistance,neoplasm;
HA117 gene
- From:
Journal of International Oncology
2011;38(8):636-639
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of HA117 in bone marrow mononuclear cells (BMMNCs) of acute leukemia patients, and its correlation with multidrug resistance( MDR), as well as its correlation with antigens presentation and its clinical significance. Methods HA117 gene expressions in 36 patients with acute myeloid leukemia (AML) and 14 patients with acute lymphocytic leukemia (ALL),10 patients with idiopathic thromnocytopenic purpura (ITP) were tested by using reverse transcriptase polymerase chain reaction (RT-PCR) technique. Thirty bone marrow specimens of AML patients at different phase were selected, and immunophenotypes were assayed by flow cytometry. ResultsThere was no significant difference ( P ＞ 0.05 ) in HA117 gene expression between ALL (78.57％) and A ML (72.22％). The expressions of HA117 gene in both ALL and AML were significantly higher than in ITP patients ( P ＜ 0.05 ). The HA117 gene was expressed in 75.00％ of the initially diagnosed patients and 77.78％ of the remission patients and 69.23％ of the nonremission patients, all of them had higher expression than the patients with ITP, but there was no significant difference among the three groups. There were 47.06％ and 87.88％ with HA117 gene expressioned in non-resistance group and resistance group respectively, and the difference between the two groups was significant(P=0.005). HA117 had positively correlation with CD11b(OR =0.59, P=0. 001 ),CD33(OR=0.704, P=0. 000) expression in 30 patients of AML, and had negative correlation with MPO (OR=－0.611, P=0.001). ConclusionHA117 gene overexpression is significantly associated with the clinical resistance. It may also be associated with MDR of acute leukemia. HA117 gene can be used as an important indicator for investigating clinical MDR and predicting prognosis of acute Ieukemia patients. HA1 17 is closely related to CD11b, CD33, MPO expression in AML, and they can be used as biomarkers for evaluating therapy efficacy in AML.
