Detection of promoter methylafion of three human leukocyte antigen (HLA) class I genes (HLA-A, -B and -C) in patients with psoriasis vulgaris
10.3760/cma.j.issn.0412-4030.2011.09.008
- VernacularTitle:寻常性银屑病患者HLAⅠ类分子A、B、C位点甲基化的研究
- Author:
Min CHEN
;
Pangen CUI
;
Lin LIN
;
Mingjun JIANG
;
Xinfeng WU
;
Wuqing ZHOU
;
Yan WANG
;
Haihong GUAN
- Publication Type:Journal Article
- Keywords:
Psoriasis;
MHC class L;
DNA methylation;
Methylation
- From:
Chinese Journal of Dermatology
2011;44(9):629-632
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the correlation between the methylation status of HLA class Ⅰ genes(HLA-A, -B and -C) in psoriatic epidermis and disease severity in patients with psoriasis vulgaris. MethodsDNA specimens were obtained from the lesional and nonlesional epidermis of 46 patients with psoriasis vulgaris and from the normal skin of 28 human controls. Methylation specific PCR (MSP) was conducted to detect the methylation status of CpG islands in the promoter region of HLA-A, -B and -C genes. The severity of psoriasis was evaluated by psoriasis area and severity index(PASI) scores. ResultsThe percentage of promoter methylation of HLA-B and HLA-C genes was 4.35％(2/46) and 21.74％(10/46), respectively in nonlesional epidermis, 4.35％ (2/46) and 4.35％ (2/46), respectively in lesional epidermis from these patients. No methylation was observed for the promoter of HLA-A, -B or -C gene in the normal control epidermis or for that of HLA-A gene in the nonlesional or lesional epidermis from the patients. The frequency of HLA-C gene promoter methylation in the nonlesional epidermis was significantly higher than that in the lesional epidermis and control epidermis, but was uncorrelated to the disease severity. No significant difference was observed for the methylation frequency of HLA-A or -B gene promoter among the three groups of specimens. Conclusion Abnormal methylation of HLA-C gene promoter is observed in patients with psoriasis vulgaris.
