Biological characteristics of rat bone marrow mesenchymal stem cells transfected with hypoxta-inducible factor-1α gene
10.3760/cma.j.issn.0254-1416.2011.07.024
- VernacularTitle:大鼠骨髓间充质干细胞经低氧诱导因子-1α基因转染后的生物学特性
- Author:
Yuming ZHANG
;
Jun WANG
;
Zhen ZHANG
;
Miaomiao Lü
;
Zhibin XIAO
;
Changjun GAO
;
Wei CHAI
;
Lixian XU
;
Xude SUN
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
Bone marrow;
Hypoxia-inducible factor 1,alpha subunit;
Transfection;
Biological characteristics
- From:
Chinese Journal of Anesthesiology
2011;31(7):858-861
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo evaluate the biological characteristics of rat bone marrow mesenchymal stem cells (BMSCs) transfected with hypoxia-inducible factor-1α(HIF-1 α) gene.MethodsThe rat BMSCs of 3rd generation and the vector expressing HIF-1α gene (pcDNA3.1-HIF-1α) were provided by department of anesthesia,Tangdu Hospital,the 4th Military Medical University.BMSCs expressing HIF-1α gene (BMSCs-HIF-1α cells) were constructed by transfection of vector pcDNA3.1-HIF-1α into BMSCs by means of electroporation.Successful transfection of HIF-1α gene was confirmed by immuno-cytochemistry.Simple BMSCs and BMSCs-pcDNA3.1 cells were used as control cells.After being cultured in hypoxic condition HIF-1α expression was detected by Western blot analysis.Flow cytometry was used to determine the proportion of cells in G1,G2 and S phase and detect apoptosis.The proliferation index (PI) was calculated.The cell growth curve was described by MTT assay and the number of the 3 types of cells was recorded.ResultsA large number of deep blue granules were observed in the nuclei of BMSCs-HIF-1α cells using immuno-cytochemistry but no such granule was found in the two types of control cells.HIF-1α expression was significantly up-regulated and apoptosis rate (the number of apoptotic cella/the total number of cells examined) decreased in BMSC-HIF-1α cells compared with the control cells.The proportion of cells in S and G2 phase was significantly higher and the proportion of cells in G1 phase was significantly lower and PI higher in BMSCs-HIF-1α cells than in the control cells.The number of BMSCs-HIF-lα cells was significantly higher than the number of the two types of control cells at day 3-8 of culture.There was no significant difference in the above variables between BMSCs and BMSCs-pcDNA3.1 cells.ConclusionBMSCs-HIF-1α is successfully constructed by transfection of vector pcDNA3.1-HIF-1α gene into BMSCs by means of electroporation.
