Application of Y chromosome specific probe to the detection of expression of endothelial progenitor cells grafted in the injured lung tissues of rats
10.3760/cma.j.issn.1671-0282.2011.11.013
- VernacularTitle:Y染色体探针示踪内皮祖细胞移植在损伤肺组织中的表达
- Author:
Mei MAO
;
Bo WANG
;
Yi WANG
;
Jiancheng XU
;
Zuhong FU
- Publication Type:Journal Article
- Keywords:
Y chromosome;
Endothelial progenitor cell;
Stem cell transplantation;
Acute lung injury;
Acute respiratory distress syndrome
- From:
Chinese Journal of Emergency Medicine
2011;20(11):1162-1166
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the efficient methods used for tracing endothelial progenitor cells(EPCs)after transplantation in injured lung tissue.Methods Thirty clean grade healthy syngeneic SD female rats were divided into three experimental groups(n =10):(1)sham group,rats treated with intravenous phosphate-buffered saline(PBS)instead of lipopolysaccharide(LPS)followed by EPCs graft;(2)PBS-treated group,rats treated with intravenous PBS after intravenous injection of LPS to produce acute lung injury;(3)EPC-treated group,rats treated with EPCs after intravenous injection of LPS to produce acute lung injury.The transplanted EPCs were got from the same genetic species of the SD male rats.Rats of each group were sacrificed 7 days after EPCs transplantation.Their whole lung tissues were harvested to detect the expression of Y-chromosome by using hybridization in situ and RT-PCR assay.Statistic package of SPSS16.0 was used for the data analysis and significant differences between means were evaluated by ANOVA analysis.Results Compared with the other two groups,positive signals of sex-determining region y were found in lung endothelium from the EPC-treated group.Conclusions Y chromosme specific probe can be one of efficient methods for tracing stem cells after transplantation.
