Effect of triiodothy ronine on neurogenesis in subventricular zone after bilateral common carotid artery occlusion in adult rats
10.3760/cma.j.issn.1673-4165.2011.08.006
- VernacularTitle:三碘甲状腺原氨酸对双侧颈总动脉闭塞成年大鼠室下区神经发生的影响
- Author:
Li TING
;
Cui DAN
;
Wang QUN
- Publication Type:Journal Article
- Keywords:
Triiodothyronine;
Thyroid hormones;
Neurons;
Stem cells;
Brain ischemia;
Rats
- From:
International Journal of Cerebrovascular Diseases
2011;19(8):589-592
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of triiodothyronine (T3) on neurogenesis in after bilateral common carotid artery occlusion in adult rats. Methods Fifteen adult male Sprague-Dawley (SD) rats were randomly divided into sham operation, 2-vessel occlusion (2VO) and T3 intervention groups (n = 5 in each group ). A chronic cerebral ischemia model was established by the bilateral common carotid artery permanent ligation. Seven days after modeling 5-bromo-2'-deoxyuridine (BrdU) was injectied intraperitoneally and the new proliferating cells were labeled. The BrdU positive cells in SVZ were observed using immunofluorescence staining.Results The numbers of BrdU positive cells in the sham operation, 2VO, and T3 intervention groups were 174. 8 ± 18. 5, 325.0 ± 51.0, and 499. 4 ± 30. 8, respectively. There were significant differences among all the groups (F = 101.68, P ＜ 0. 001 ). The numbers of BrdU positive cells in the T3 intervention group were significantly higher than those in the 2VO and sham operation groups (all P ＜0. 001 ). The numbers of BrdU positive cells in the 2VO group were also significantly higher than those in the sham operation group (p ＜0. 001). Conclusions Thyroid hormones may promote neural progenitor cell proliferation in SVZ in adult 2VO rats.
