Evaluation of myocardial viability during cold storage by measurement of myocardial dielectric properties tanδm in radio frequency
10.3760/cma.j.issn.0254-1785.2011.09.013
- VernacularTitle:心肌组织射频频段介电特性参数tanδm在保存猪心心肌活性评价中的应用
- Author:
Guixin DUAN
;
Xuegang LIU
;
Guowen WANG
;
Ge LIU
;
Lei ZHANG
;
Yin LIANG
;
Kangwu WANG
;
Ansheng WANG
;
Chao SHI
;
Qichao LIANG
- Publication Type:Journal Article
- Keywords:
Swine,Miniature;
Myocardium;
Cryopreservation;
Dielectric property
- From:
Chinese Journal of Organ Transplantation
2011;32(9):558-561
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the mechanism of myocardial dielectric property changes in radio frequency during hypothermic preservation and explore myocardial viability evaluative method. Methods Hybrid young pigs (20-30 kg) were used in the experiment. Heart arrest was induced with GIK solution. According to preservative temperature, the animals were divided into three groups: group A (4 ℃),group B (15 ℃) and group C (25 C). The heart was preserved in saline for 12,6 and 4 h respectively. Myocardial dielectric properties and ATP content were tested every 5 and 30 min during hypothermic preservation respectively. The relationship between tanoδm and ATP content was analyzed. ResultsTanδm of three groups was decreased significantly at the beginning, most slightly in group A and most obviously in group C. There was no significant difference in Tanδm between group B and group C with group A at 1 h,but there was obvious difference at 2,3 and 4 h (P＜0. 05,P＜0. 05,P＜0. 01 ). There was correlation between tanδm and ATP in the three groups. ConclusionTanδm in three groups is dropped with preservation time and temperature, and tanδm had a correlation with ATP content. Detection of myocardial dielectric property in radio frequency is a kind of quick, accurate and noninvasive method.
