Morphological changes of pancreatic cancer cells transfected by dendrimer modified carbon nanotubes
10.3760/cma.j.issn.1674-1935.2011.04.012
- VernacularTitle:树形分子修饰的碳纳米管颗粒转染胰腺癌细胞BxPC3安全性的研究
- Author:
Dongfeng CHENG
;
Xubo WU
;
Jiaxiang WANG
;
Boosan HAN
;
Xueqing ZHANG
;
Hao YANG
;
Hengguang YUAN
;
Ping ZHU
;
Chenghong PENG
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
Carbon nanotubes;
Dendrimer;
Transmission electron microscopy;
Vector
- From:
Chinese Journal of Pancreatology
2011;11(4):269-271
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the process of polyamidoamine dendrimer modified single walled carbon nanotubes complexes (CNT-PAMAM-D) entering into pancreatic cancer BxPC3 cells, and to evaluate its safety as a vector. Methods The CNT-PAMAM-D were prepared by ultrasound, mixing and rinsing, and the morphology was characterized by atom force microscopy and transmission electron microscope. Then the prepared CNT-PAMAM-D was incubated with human pancreatic cancer cell line BxPC3 cells for 12, 48, 72hours, and then the cells were collected. Distribution of CNT-PAMAM-D in cells and cell ultrastructure was observed by transmission electron microscopy. Results After the combination of PAMAM-D and CNT, the surface of CNT was surrounded by dendrimer and nanocomposite particles with a size of 20 nm was formed. It was showed that CNT-PAMAM-D could be transfected into human pancreatic cancer cells by cell pinocytotic way, and it entered cytoplasm at 12 h. With the extension of the transfecting time, CNT-PAMAM-D could enter lysosomal and nucleus, but the morphology and ultrastructure ocf BxPC 3 cells was not significantly changed. Conclusions The CNT-PAMAM-D is a highly effective and safe nano vector.
