Small interfering RNA in silencing Bcl-2 expression and enhancing radiosensitivity of esophageal cancer cells
10.3760/cma.j.issn.1004-4221.2011.05.021
- VernacularTitle:小干扰RNA沉默Bcl-2表达增强食管癌细胞放射敏感性研究
- Author:
Dongmei LIU
;
Guowen LI
;
Xifeng TIAN
;
Songhui ZHANG
- Publication Type:Journal Article
- Keywords:
Ribonucleic acid interference;
Radiosensitivity;
Bcl-2 expression;
Cell lines,esophageal neoplasms
- From:
Chinese Journal of Radiation Oncology
2011;20(5):435-437
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the effects of small interfering RNA (siRNA) specific to Bcl-2gene on radiosensitivity of esophageal cancer cells. Methods Bcl-2 gene siRNA ( Bcl-2 siRNA ) was induced into esophageal cancer EC9706 cells by lipofectamine.Bcl-2 protein expression and apoptosis of EC9706 cells were detected by flowcytometer. Clone forming assay was used to determine the inhibitory effects of X-ray radiation combined with Bcl-2 siRNA interference. ResultsWhen Bcl-2 siRNA had been induced into EC9706 cells, Bcl-2 protein expression in EC9706 cells was inhibited, and cell apoptosis was increased. Bcl-2 protein expression rates of EC9706 cells induced with Bcl-2 siRNA1, A2, A3 (25.13% ±2. 04% ,38.87% ± 3.34% , 30.55% ± 2. 73% ) were lower than the control group ( 84.28% ± 1. 47% )(t =4. 01,3.04,3.64, P < 0. 05 ). After interference, the apoptosis rate of EC9706 cells ( 33.86% ±1.04% ) was higher than the control group and siRNA negative group (5.51% ±0. 14% and 5.59% ±0. 46% ) (t =6. 55,6. 54,P <0. 01 ). Bcl-2 gene siRNA interference enhanced X-ray inducing apoptosis of EC9706 cells (56.76% ± 1.24% ), which was higher than the radiation alone group ( 24.51% ± 0. 48% )(t =3.59,P < 0. 05 ). The D0, Dq, and SF2 of combined treatment group were much lower than those of irradiation alone group . The sensitization enhancing ratio was 1.32 ( ratio of D0 values ) . Conclusions Bcl-2 gene siRNA could enhance the radiosensitivity of esophageal cancer EC9706 cells and may has a good future in clinical practice.