Application of glucose-6-phosphate isomerase and auto-antibodies in diagnosis of rheumatoid arthritis
10.3760/cma.j.issn.1671-7368.2011.09.020
- VernacularTitle:葡萄糖-6-磷酸异构酶抗原及相关自身抗体在类风湿关节炎诊断中的价值
- Author:
Yanyan SUN
;
Hongyu DONG
;
Liang ZHANG
;
Xuemei WU
;
Yingjiu ZHANG
;
Ye LI
;
Yan HE
- Publication Type:Journal Article
- Keywords:
Arthritis,Rheumatoid;
Enzymes;
Diagnosis
- From:
Chinese Journal of General Practitioners
2011;10(9):664-666
- CountryChina
- Language:Chinese
-
Abstract:
Blood samples were collected from 90 patients with confirmed rheumatoid arthritis (RA), 73 patients with other rheumatic diseases and 50 normal controls. Serum glucose-6-phosphate isomerase (GPI) and anti-cyclic citrullinated peptide (CCP) were detected with ELISA method, rheumatoid factor IgM( RF IgM)was measured with turbidimetric immunoassay and antikeratin antibody (AKA) was determined with indirect immunofluorescence assay. The serum GPI levels of RA group[1.73 (0. 43 -4. 40)mg/L]were significantly higher than those of rheumatic disease[0. 14 (0. 10 -0. 18 )mg/L]and normal controls[0. 12 (0. 09 - 0. 15 ) mg/L], ( H = 18. 13, P ＜ 0. 01 ). The sensitivities of GPI, anti-CCP,RF-IgM, AKA, GPI + CCP, GPI + RF-IgM were 80% (72/90), 57% (51/90), 68% (61/90), 29% (26/90) ,92% (83/90) and 96% (86/90), respectively; the specificities were 97% (119/123) ,95% ( 117/123 ) ,77% (95/123) ,95% ( 117/123 ), 93% ( 115/123 ) and 76% ( 93/123 ), respectively. GP1 level was significantly correlated with CCP and RF-IgM ( r = 0. 674 and 0. 533, P ＜ 0. 01 ), but not correlated with AKA ( r = 0. 12, P ＞ 0. 05 ). The results suggest that serum glucose-6-phosphate isomerase level is a valuable indicator for early diagnosis of rheumatoid arthritis.
