Compulsive drug-seeking motivation in rats provoked by escalating high-dose morphine pretreatment and the dopaminergic and nondopaminergic neuron activity in midbrain
10.3760/cma.j.issn.1674-6554.2011.08.002
- VernacularTitle:吗啡诱发的大鼠强迫性觅药动机及中脑神经元活动性改变
- Author:
Yonghua PENG
;
Yunjing BAI
;
Jing LIANG
;
Xigeng ZHENG
- Publication Type:Journal Article
- Keywords:
Compulsive drug-seeking;
Morphine;
Addiction;
Substantia nigra pars reticulate
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2011;20(8):677-680
- CountryChina
- Language:Chinese
-
Abstract:
Objective To probe the different expression of psychomotor in different model of pretreatment and the influence of pretreatment context on the expression of psychomotor.And to compare dopaminergic and non-dopaminergic neuron activity in midbrain after different pretreatment.Methods Rats were pretreated by escalated high-dose morphine( HD group) ,fixed low-dose morphine( LD group) and saline as control(S group).After withdrawal, rats were challenged by morphine in non-drug used environment.Locomotor behavior and stereotypy behavior were monitored as the index of compulsive drug-seeking motivation.By double labeling immunohistochemistry of tyrosine hydroxylase (TH) and Fos protein, the plasticity of neuronal activity in ventral tegmental area and substantia nigra of rats response to morphine challenge were disclose after pretreated with morphine or saline.Results After 10 mg/kg morphine challenge in non-drug used environment,the locomotor behavior and the stereotypy behavior of HD group were both significant higher than that of S group ( Locomotor behavior and Stereotypy behavior,HD group: 26907 ± 2003 and 129.6 ± 6.5; S group: 14962 ± 1888 and 89.9 ± 10.9, all P ＜ 0.01 ).The results of immunohistochemistry showed that Fos protein expressed in the SNr and in lateral SNr was significantly higher in the HD group compared to S groups ( Fos expression in SNr and lateral SNr,HD group: 29.14 ±5.27 and 9.83 ± 2.84; S group: 17.29 ± 1.51 and 2.06 ±0.45; all P＜0.05 ).Conclusion The sensitization behavior expression of the rat pretreated by escalated high-dose morphine is not modulated by drug using enviroment compared with fixed low-dose morphine pretreatment.The neuroplasticity of SNr,especially lateral SNr might be one of the mechanisms underlying the uncontroled sensitization behavior expression.
