Inhibition effect of sodium demethylcantharidate injection on adherence, invasion and metastasis of human hepatocarcinoma HepG2 cells
10.3760/cma.j.issn.1006-9801.2012.10.009
- VernacularTitle:去甲斑蝥酸钠注射液对人类肝癌HepG2细胞侵袭迁移、黏附的抑制作用
- Author:
Haikun YANG
;
Huiping LIU
;
Haoliang ZHAO
- Publication Type:Journal Article
- Keywords:
Hepatocarcinoma HepG2 cells;
Sodium demethylcantharidate injection;
Metastasis;
Adherence;
Invasion;
Matrix metalloproteinase 9
- From:
Cancer Research and Clinic
2012;24(10):678-681
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of sodium demethylcantharidate injection on adherence,invasion and metastasis and to investigate the related mechanism in human hepatocarcinoma cell line HepG2.Methods Adherence ability,migration and invasion of HepG2 cells inhibited by sodium demethylcantharidate injection were assessed by MTT and Transwell techniques.Expression levels of MMP-9 protein in HepG2 cells were determined by immunohistochemistry.Results The number of adhesion,migration and invasion of HepG2 cells were significantly lower in sodium demethylcantharidate injection group than those in the control group (P ＜ 0.05).HepG2 cells co-incubated with sodium demethylcantharidate injection in the concentration of 0.25 μg/ml for 30,60,90 and 120 min showed higher cell adhesion than the control group.The adhesion inhibition ratios were 48.11％,33.81％,28.97 ％ and 16.83 ％,respectively.The migration and invasion inhibition rates were 64.19 ％ and 58.19 ％.With concentration of sodium demethylcantharidate injection to increasing,expression levels of MMP-9 protein in HepG2 cells more and more lower than control group.Conclusion The adherence,migration and invasion abilities of HepG2 cells are markedly inhibited by sodium demethylcantharidate injection,the mechanisms is possible related to the expression levels of MMP-9 protein.
