Clinical observation of cytokine-induced killer cells combined with chemotherapy on the treatment of advanced colorectal cancer
10.3760/cma.j.issn.1006-9801.2012.10.005
- VernacularTitle:细胞因子诱导的杀伤细胞联合化疗治疗晚期结直肠癌的临床观察
- Author:
Zengqing GUO
;
Yunbin YE
;
Jiami YU
;
Xiaojie WANG
;
Mingshui CHEN
;
Xiurong CHEN
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Cytokine-induced killer cells;
Drug therapy;
Adoptive cellular immunotherapy
- From:
Cancer Research and Clinic
2012;24(10):663-666
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the clinical effects of cytokine-induced killer (CIK) cells combined with chemotherapy on the treatment of patients with advanced colorectal cancer.Methods CIK cells were prepared from 50 ml peripheral blood mononuclear cells by stimulated with IL-2,IFN-γ,anti-CD3 monoclone antibody,IL-1 for 8 d.The clinical effects and survival rate were compared between CIK cells combined with chemotherapy group and the chemotherapy group (50 patients with advanced colorectal cancer for each).T cells and NK cells of patients were tested by FCM before and after CIK cells treatment.The improvement of quality of life and toxicity of this therapy were observed.Results The percentages of CD3+,CD4+,CD8+ T cells and NK cells were (54.779±14.228) ％,(30.821±11.554) ％,(16.676±6.256) ％,(18.705±9.347) ％ before CIK cells transfusion.After transfusion,the percentages were (65.236±14.901) ％,(37.292±8.880) ％,(25.229±6.711) ％,(22.950±8.933) ％,respectively.The percentages were expanded greatly (P ＜ 0.05).The patients quality of life were improved clearly with lower toxicity.The DCR of CIK cells combined with chemotherapy group (64 ％,32/50) was higher than the chemotherapy group (40 ％,20/50) (P ＜ 0.05).The survival rate between two groups had no statistical significance (P ＞ 0.05).Conclusion Administration of CIK cells combined with chemotherapy can enhance immune function in patients with advanced colorectal cancer and improve their quality of life,and get good clinical efficacy.
