Detection and clinical significance of Th17 cell proportion and expression of IL-17 in peripheral blood of patients with non-small cell lung cancer
10.3760/cma.j.issn.1006-9801.2012.09.007
- VernacularTitle:非小细胞肺癌患者外周血Th17细胞比例和血清IL-17水平的检测及其临床意义
- Author:
Shuqiang ZHAO
;
Chunsheng JIA
;
Guiqin WANG
;
Shuqin LI
- Publication Type:Journal Article
- Keywords:
Carcinoma,non-small cell lung;
Flow cytometry;
Th17 cells;
Interleukin-17
- From:
Cancer Research and Clinic
2012;24(9):600-602
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between the proportion of Th17 cells and IL-17 expression in the peripheral blood and their clinical significance in patients with non-small cell lung cancer (NSCLC).Methods The proportion of Th17 cells determined by flow cytometry (FCM) and IL-17 detected by enzyme-linked immunosorbent assay (ELISA).The data was analyzed from 60 untreated patients with NSCLC (NSCLC group) and 20 healthy volunteers (control group).Results The Th17 cells proportion in CD+3 T-cells in NSCLC group was significantly higher than that in control group [(1.23±0.41) ％ vs (1.05±0.28) ％,t =1.679,P =0.097].The expression levels of Th17 cells in peripheral blood of patients with squamous cell carcinoma was significantly higher than that in adenocarcinoma patients [(1.31±0.39) ％ vs (1.09±0.41) ％,t =2.093,P =0.041].The concentration of IL-17 of patients with NSCLC was significantly higher than that in control group [(21.11±7.87) pg/ml vs (17.64±5.07) pg/ml,t =2.280,P =0.027].The expression levels of serum IL-17 of squamous cell carcinoma patients was significantly higher than that of adenocarcinoma patients [(23.11±8.73) pg/ml vs (18.54±6.38) pg/ml,t =2.203,P =0.032].As clinical stage progressed,the proportion of Th17 cells and IL-17 expression were significantly downtrend [F =3.151,P =0.032,F =4.132,P =0.010].Conclusion The levels of Th17 cells and IL-17 in the peripheral blood of patients with NSCLC significantly correlated with the pathological types and TNM stage,might play an important role in the course of the disease.
