Biological variations of serum lipoprotein subfractions and high-density lipoprotein cholesterol esterification rate
10.3760/cma.j.issn.1009-9158.2011.12.021
- VernacularTitle:血清脂蛋白亚类和高密度脂蛋白胆固醇酯化速率的生物学变异
- Author:
Hanbang GUO
;
Hongxia LI
;
Yong MAN
;
Shu WANG
;
Jun DONG
;
Wenxiang CHEN
- Publication Type:Journal Article
- Keywords:
Lipoproteins,HDL;
Lipoproteins,LDL;
Cholesterol,HDL;
Esterification;
Variation(genetics)
- From:
Chinese Journal of Laboratory Medicine
2011;34(12):1135-1138
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the biological variations ( CVI,CVG ) of serum high-density (HDL2-C,HDL3-C ) and low-density (LDLa-C,LDLb-C ) lipoprotein subfractions and high-density lipoprotein cholesterol esterification rates (FERHDL and MERHDL ).MethodsTwenty healthy volunteers,10 males and 10 females,were recruited for this study from September to October,2010.Blood was collected four times from each individual with a 2-week interval between each sampling.Serum lipoprotein subfraction cholesterol levels were measured by ultracentrifugation/HPLC,FERHDL and MERHDL were measured by HPLC.Within-subject ( CVI) and between-subject ( CVG.) biological variations and quality specifications for precision,bias and total error were calculated.Results The average CVI of this group were 5.5％ and 7.2％ for HDL3-C and HDL2-C,11.2％ and 18.7％ for LDLa-C and LDLb-C,11.95％ and 12.3％ for FERHDL and MERHDL,respectively.The CVG for HDL2-C was 45.5％,much higher than that of HDL3-C (8.7％),and FERHDL(49.5％ ) had a higher CVG than MERHDL (30.6％ ).For each analyte,there was a considerable variation of CVIamongindividuals.ConclusionsBiologicalvariationsof lipoprotein subfractions,FERHDI and MERHDL have been estimated.These rsults will play an important role in quality specifications and cardiovascular disease risk assessment.
