Identification of Peudallescheria boydii/Scedosporium apiospermum dinical isolates by sequence differences of ITS and TUB
10.3760/cma.j.issn.1009-9158.2011.12.007
- VernacularTitle:用ITS和TUB序列差异鉴定临床相关波氏假阿利什霉/尖端赛多孢
- Author:
Sibu WANG
;
Jie ZHANG
;
Jin YU
;
Shaojie CHEN
;
Zhe WAN
;
Ruoyu LI
- Publication Type:Journal Article
- Keywords:
Pseudallescheria boydii/ Scedosporium apiospermum;
rDNA;
Tubulin
- From:
Chinese Journal of Laboratory Medicine
2011;34(12):1084-1087
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTore-identifyfifteenclinical Pseudallescheriaboydii/Scedosporium apiospermum isolates by the sequence difference of ITS rDNA and partial β-tubulin gene (TUB) and thus understand thepathogenicstrain typesfor guiding theclinicaltreatment.MethodsMorphological appearances,D-ribose assimilation and sequencing of ITS and TUB were used to re-identify the fifteen clinical strains of Pseudallescheria boydii/Scedosporium apiospermum.The sequences of ITS and TUB were analyzed with Clustal X and MEGA 4 software.Results No difference of morphological appearances was found in the fifteen strains.Cleistothecium was observed in one isolate.All the strains were D-ribose assimilation positive.The clinical strains were re-identified as P.boydii species complex by the CBS database (http://www.cbs.knaw.nl).ElevenstrainswereP.boydiiandfourstrainswereS.apiospermum respectively.Conclusions P.boydii and S.apiospermum cannot be identified correcdy by the time-consuming conventional morphological method and biochemical characteristics.The study recommend that the clinical isolates of P.boydii and S.apiospermum should be identified utilizing a combination of traditional phenotype method and molecular biotechnology.
