The analysis of genetic polymorphisms and haplotype of the Trail gene in the patients with ulcerative colitis
10.3760/cma.j.issn.0254-1432.2011.12.006
- VernacularTitle:溃疡性结肠炎患者中肿瘤坏死因子相关凋亡诱导配体基因多态性及单倍型分析
- Author:
Yi JIANG
;
Jihua PEI
;
Limiao LIN
;
Changlong XU
;
Jianzhang WANG
;
Sujian SHEN
;
Zhanxiong XUE
- Publication Type:Journal Article
- Keywords:
Colitis,ulcerative;
Apoptosis;
Tumor necrosis factor;
Ligands;
Polymorphism,genetic;
Haploidy
- From:
Chinese Journal of Digestion
2011;31(12):812-816
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the association between genetic polymorphisms and haplotypes of tumor necrosis factor-related apoptosis inducing ligand (Trail) and ulcerative colitis (UC).Methods A total of 331 patients with UC and 832 age and sex-matched healthy controls were collected.After Trail gene was amplified by PCR,the genetic polymorphisms of three single nucleotides (G1525A/G1588A/C1595T) in 3' non coding regions of Trail gene were examined by direct sequencing.The relation between Trail haplotype and UC was analyzed.Results Compared with control group,the frequencies of variant allele A and genotype GA+ AA in Trail G1525A were significantly lower in UC group (both P＜0.01).The frequencies of variant allele A and T in Trail G1588A and C1595T were also significantly lower in UC group than that of control group,and the difference was statistically significant (both P ＜ 0.01 ).In mild and moderate UC patients,the frequencies of variant allele T and CT+TT in Trail C1595T were 49.15％ and 64.51％,in severe UC patients were 72.37％ and 84.21％,and the differences were significant between the two groups (OR=2.710 and 2.935,95％CI:1.598～4.596 and 1.188～7.249,all P ＜0.05).In severe UC patients,the frequency of variant allele A in Trail G1525A was 48.69％,which was higher than that of mild and moderate patients (35.16％,OR=1.750,95％CI:1.082～2.830,P=0.021).In UC group,the frequency of AAT haplotype was significantly lower than that of controls (43.09％ vs 58.41％,P＜0.01).The frequency of GAT haplotype was significantly higher in UC group (10.15％vs 0.18％,95％ CI:0.005 ～ 0.051,P＜ 0.01).Conclusion The genetic polymorphisms and haplotypes of Trail (G1525A/G1588A/C1595T) gene may be closely correlated with the susceptibility to UC.
