The significance of human feces Vimentin gene methyaltion analysis in early diagnosis of colorectal cancer
10.3760/cma.j.issn.0254-1432.2011.12.003
- VernacularTitle:人粪便中波形蛋白基因甲基化在结直肠癌早期诊断中的意义
- Author:
Qiongqiong ZHU
;
Jiansheng LI
;
Jinping ZHANG
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Vimentin;
Methylation;
Polymerase chain reaction;
Feces
- From:
Chinese Journal of Digestion
2011;31(12):803-807
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the methylation status of Vimentin gene in feces of colorectat cancer (CRC) patients,to explore the possibility and clinical significance of which as molecular marker for early diagnosis of colorectal cancer.Methods DNA was isolated in feces of 60 colorectal cancer patients hospitalized and treated in the First Affiliated Hospital of Zhengzhou University,17 colorectal adenoma patients underwent colon endoscopy in outpatient department and 30 normal volunteers.The methylation status of Vimentin gene was analyzed with methylation-specific PCR (MSP).Results In the feces of 60 colorectal cancer patients,the positive percentage of Vimentin gene methylation was 51.67％ (31/60).In the feces of 17 colorectal adenoma patients,the positive percentage of Vimentin gene methylation was 4/17.Vimentin gene methylation was detected in none of 30 normal controls.Conclusion The methylation of Vimentin gene in fecal is an early event in colorectal cancer progression.Analysis of Vimentin gene methylation in feces is expected to be one of the markers for early diagnosis of colorectal cancer.
