Study on MICA/B gene polymorphism in northern Hunan Han population

VernacularTitle:湖南北部汉族人群MICA/B基因多态性研究
Author: Zheng GONG ; Haibo PENG ; Yuping SU ; Qizhi LUO ; Lin LIN ; Ping YU
Publication Type:Journal Article
Keywords: HLA; MICA/B; PCR-SBT; PCR-SSP; Hunan Han population; Gene polymorphism
From: Chinese Journal of Microbiology and Immunology 2012;32(9):785-791
CountryChina
Language:Chinese
Abstract: Objective To study on the characterization of MICA/B genetic polymorphism in a northern Chinese Hunan Han population.Methods Ninty-five unrelated individuals were involved in this study and MICA/B genotypes were determined by two methods:PCR-sequence-specific primers (PCR-SSP) and PCR-sequence-based typing (PCR-SBT).Results In northern Hunan Han population,eleven MICA alleles were found,among which MICA * 010 (28.95％),MICA * 008 ∶ 01 (20.53％) and MICA * 002 ∶ 01 (15.79％) were the common alleles.Five MICA-STR(short tandem repeat) alleles were found,among which MICA * A5 (37.89％) and MICA * A5.1 (21.05％) predominated.In this population,ten MICB alleles were found.The common alleles were MICB * 005 ∶ 02/* 010 (58.42％),MICB * 002 ∶ 01 (10.00％),and MICB * 008 (7.89％).Two kinds of MICA-MICB haplotypes were MICA * 004-MICB * 004 ∶ 01 and MICA * 010-MICB * 005 ∶ 02/010 in significant linkage disequilibrium.This study also showed MICA/B gene with high polymorphism in different populations.Conclusion MICA/B alleles distribution in northern Hunan Han population with its unique characteristics.