HLA-A, B, and DRB1 polymorphism at high-resolution in Ji-nan Han population

VernacularTitle:济南汉族人群HLA-A、B、DRB1等位基因高分辨多态性分析
Author: Chuanxing ZHANG ; Lu WANG ; Yi ZHANG ; Chuanfu ZHU
Publication Type:Journal Article
Keywords: Human leukocyte antigen; Polymorphism; Han population
From: Chinese Journal of Microbiology and Immunology 2012;32(8):685-688
CountryChina
Language:Chinese
Abstract: Objective To analyze the polymorphic character of HLA-A,B,DRB1 alleles at highresolution level in Ji-nan Han population.Methods A total of 483 unrelated and healthy Han ethnic individual from Ji-nan region were genotyped by PCR-SBT for HLA-A,B and DRB1 loci.Then allelic and haplotypic frequencies were calculated by maximum likelihood estimation method using Arlequin software.Results There were 27,56 and 41 alleles identified at HLA-A,B and DRB1 loci respectively,and the most common alleles were A * 11∶01(0.1615),B * 13∶02(0.1163) and DRB1 * 07∶01 (0.1763).The three loci haplotype was dominated by A* 30∶01-B * 13∶02-DRB1 * 07∶01 with a frequency of 0.0867.Conclusion The HLA-A,B and DRB1 loci are highly polymorphic in Ji-nan Han population.