The establishment of the mouse congenital infection model by MCMV and the observation of the nervous system infection
10.3760/cma.j.issn.0254-5101.2012.08.002
- VernacularTitle:小鼠MCMV先天感染模型的构建及神经系统感染状况的观察
- Author:
Xiaoyi DU
;
Yufeng ZHOU
;
Xinglou LIU
;
Sainan SHU
;
Feng FANG
- Publication Type:Journal Article
- Keywords:
Cytomegalovirus;
Animal model;
Congenital infection;
Nervous system;
Brain tissue
- From:
Chinese Journal of Microbiology and Immunology
2012;32(8):679-684
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the murine congenital infection model by MCMV and observe the pathological changes and infection status of brain tissue.Methods After anesthesia,mice who were pregnant 11-13.5 days (E11-13.5 d) were intra-amniotic injected one uterus by one with virus (MCMV K181 suspension,1 μl,1×103 PFU).The control group of the same period was intra-anmiotic injected with culture medium DMEM (1 μl).Carefully reset the uteruses and close the abdomen.After 5 days of separated feeding,kill the pregnant mice,take the fetus out of the uterus,anesthetize and kill them.Make frozen sections of these fetal brains.Some sections were stained using conventional HE method,to observe the pathological changes under the light microscope.Detect MCMV early antigen in the brain tissue by immunohistochemistry staining and immunofluorescence assay.Results The survival rates of the infected group were 71.9％.Compared with the control group,intra-amniotic inoculation of MCMV does not affect the rate of fetal survival,fetal absorption,fetal death and the average weight of the heads,but decrease their average weight of the bodies.The pathological changes are found in the brain tissue of the mouse in the infection group.Through enzyme immunohistochemistry assay,there are many MCMV infected cells in brain-ventricular zone,brain subependymal zone,cerebral cortex and hippocampus area in the infection group.Similar findings were observed by immunofluorescence method.Conclusion By intra-amniotic injection of MCMV suspension,murine model of MCMV congenital infection can be successfully established.This model could be used to study the mechanisms of encephalodysplasia caused by congenital CMV infection in vivo.
