The increased express of TIM-1, TIM-3 and other association genes in peripheral blood mononuclear cells from patients with Graves disease
10.3760/cma.j.issn.0254-5101.2012.07.018
- VernacularTitle:Graves病患者外周血单个核细胞TIM-3、TIM-1及其相关基因mRNA的表达与临床意义
- Author:
Tao LIANG
;
Xiaobei WANG
;
Pengcheng CAI
;
Lihua HU
- Publication Type:Journal Article
- Keywords:
Graves disease;
TIM-3;
TIM-1;
Th1/Th2
- From:
Chinese Journal of Microbiology and Immunology
2012;32(7):647-651
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the levels of the mRNA expression of TIM-3,TIM-1,T-bet,GATA-3,IFN-γ,IL-4 and Galectin-9 in the peripheral blood monocytes (PBMCs) of the patients with Graves disease(GD),and to explore their potential role in the pathogenesis of GD.Methods We used fluorescence quantitative real-time reverse transcription-polymerase chain reaction to measure the mRNA expression of TIM-3,TIM-1 and other associated genes in PBMCs of 70 patients with GD and 22 healthy controls.In addition,we analyzed the relationship of TIM-3,TIM-1 and other associated genes.Results The expression of TIM-3 and TIM-1 mRNA in the PBMCs from GD patients were abnormally higher,the GD patients with Graves' ophthalmopathy group had significantly higher level of TIM-3 mRNA expression than that of GD patients without Graves' ophthalmopathy group,but no statistically significant difference was found in the expression of TIM-1 mRNA.Untreated GD patients had significantly higher level of TIM-3 mRNA expression than that of GD patients in recurrence group,however the expression of TIM-1 mRAN was opposite.But no statistically significant difference was found in TIM-3 mRNA expression of recovery GD patient and healthy control group.Though the expression of TIM-1 mRNA was significantly decreased,it was still higher than that of the normal control group.Conclusion TIM-3 and TIM-1 may participate in the occurrence,development and turnover of GD.TIM-3 or TIM-1 may prove to be an important target for developing new drugs and treatments to GD.
